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AppliedBiosystems7500RealTimePCRmanual_c

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Applied Biosystems 7500 Real-Time

PCR System

SDS 1.4

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Allelic Discrimination

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12. brunningLAYQW1~20cycle (¥bcycle¼Æ¡A¨C-Ó3cyclee

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11

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wb

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hXUCAihGAMU

12

3. FAmplification Plot~GiG

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Spectra: N

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13

Standard Curve: J~qneXuA~A~AiIkOmit WellsN

Outlier~RC

14

Dissociation: YHTwS

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Dissociation Curve

non-specificCpUG

Ynon-specificAGpUG

15

Report: psisExceliAC*.csvAYLBib

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16

IV. Relative Quantification µGR

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17

3. OiiRRsingle plateOPihplateJplatehnWL

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sample name¡C

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a. ThresholdBaselinew

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18

b. Gene Expressionw

~WAWCt~Ai5. wnRAUR: bCalibrator SampleBIEndogenous Control DetectorRQ Min/MaxpAU

iR

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19

7. qGene ExpressioniowqGIweApBars¡C

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8. IFile/Export/Results/BothNGsExcel(*.csv)

20

V. ~

iKstXKs

(8 Tubes/Strip)Optical Tubes (P/N4316567, 125 strips)(8 Caps/Strip)Optical Caps(P/N 4323032, 300 strips)»

21

VI. ªN:

96 well optical plate¡BKsoptical tube¡AtXA

PCR block

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run

background plate(8 tubes/strip) precision plate

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Hupdate background file¡C 5. Nq

: 0800251326

http://www.appliedbiosystems.com.tw

22

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